As the Oprah Winfrey show sunsets after 25 years on air, comedians on Twitter took to poking fun at the Queen of Talk.

The daytime diva was the butt of numerous jokes, with Today's Top Tweets featuring quips by Jason Maybe, Neil Mayhem, Thomas Towell, Alex Baze, Mike Birbiglia, Joe Veix, Jonathan Daniel Brown, Lifecoachers, Guy Nicolucci and Rob Huebel.